Revolutionizing aptamer-graphene oxide biosensing: detection, therapeutic applications and environmental monitoring from a clinical perspective
Abstract
The increasing use of bioinoculants to enhance soil quality and crop productivity requires efficient sensor platforms for fast monitoring. EU and national registration demand analytical methods to evaluate environmental fate and long-term effectiveness. Addressing these challenges requires evaluating bioinoculant persistence and impact on microbial communities, emphasizing the need for reliable detection methods in soi (…)
Graphical abstract
This study presents a novel QCM-D aptasensor for detecting Bacillus subtilis in soil, utilising ssDNA aptamers for high specificity and sensitivity. Tested on Polish and German soils, the sensor maintained robust performance despite complex matrices, achieving a detection limit between 105 and 106 CFU/ml. The platform’s adaptability makes it promising for real-time biofertilizer monitoring and agricultural applications.
This study presents a novel QCM-D aptasensor for detecting Bacillus subtilis in soil, utilising ssDNA aptamers for high specificity and sensitivity. Tested on Polish and German soils, the sensor maintained robust performance despite complex matrices, achieving a detection limit between 105 and 106 CFU/ml. The platform’s adaptability makes it promising for real-time biofertilizer monitoring and agricultural applications.
